YPet
Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm.
Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.
Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (defined as the product of the extinction coefficient and quantum yield). Typically, YFP serves as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is monomeric cyan fluorescent protein (mCFP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y substitution introduced by mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
"Venus" contains a novel amino acid substitution –F46L– which accelerates the oxidation of the chromophore at 37°C, the rate limiting step of maturation. The protein has other substitutions (F64L/ M153T/ V163A/ S175G), permitting Venus to fold well and giving it relative tolerance to acidosis and Cl-.

This is an excerpt from the article YPet from the Wikipedia free encyclopedia. A list of authors is available at Wikipedia.
The article YPet at en.wikipedia.org was accessed 10 times in the last 30 days. (as of: 06/09/2013)
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YPet Sequence and Map - SnapGene
Yellow fluorescent protein optimized for FRET with CPet.
www.snapgene.com/resources/plasmid_files/fluorescent_protein_genes_and_plasmids/YPet/
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Addgene - YPET-GGA3 Plasmid Data
The fluorescent protein YPet was added to the amino terminus of amino acids 148-303 from GGA3. Vector backbone: pEF4-myc-HisA (Search Vector Database ) ...
www.addgene.org/18841/
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Addgene - pBabe-Ypet-PBD Plasmid Data
Plasmid 24533: pBabe-YPet-PBD. Gene/insert name: ... Fusion protein or tag: YPet. Terminal: N terminal on insert. Vector backbone: pBabe-sin-tet-CMV-puro ...
www.addgene.org/24533/
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Advances in fluorescent protein technology
Dec 15, 2007 ... Many of the cyan and yellow FP mutations introduced near the termini of the proteins resulted during the CYPet and YPet mutagenesis efforts ...
jcs.biologists.org/content/120/24/4247.long
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Experiment ~ ECFP/YPet is the best FRET pair for live cell imaging ...
Nov 5, 2008 ... ECFP/YPet is the best FRET pair for live cell imaging, however the high sensitivity replies on the dimerization of the fluorescent proteins.
en.dogeno.us/2008/11/ecfpypet-is-the-best-fret-pair-for-live-cell-imaging-but-the-high-sensitivity-replies-on-the-dimerization-of-the-fluorescent-proteins/
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YPET Spring 2012 | The Eastern Tibet Training Institute
Through its Spring 2012 Youth Pre-Employment Training (YPet) class, ETTI recruited, trained and graduated 28 students. The class comprised students from ...
www.etti.org.cn/youth-pre-employment-training/ypet-spring-2012/
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Yellow fluorescent protein - Wikipedia, the free encyclopedia
Three improved versions of YFP are Citrine, Venus, and YPet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the ...
en.wikipedia.org/wiki/Yellow_fluorescent_protein
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An experimental study of GFP-based FRET, with application to ...
We suspected that dimerization of the YPet–CYPet might be the problem, so we introduced the monomerizing mutation A206K (Zacharias et al. 2002), first in ...
www.ncbi.nlm.nih.gov/pmc/articles/PMC2206698/
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FlyBase Recombinant Construct Report: P{UAS-YPEt-Zyx}
Symbol, P{UAS-YPet-Zyx}, FlyBase ID, FBtp0041103. Feature type, transgenic_transposon. Size, Expression data. Associated insertions, 0 available. Molecular ...
flybase.org/reports/FBtp0041103.html
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Evolutionary optimization of fluorescent proteins for intracellular ...
Feb 6, 2005 ... The resulting pair, CYPet-YPet, exhibited a 20-fold ratiometric FRET signal ... The CYPet-YPet energy transfer pair provides substantially ...
www.nature.com/nbt/journal/v23/n3/abs/nbt1066.html
Search results for "YPet"
Google: approx. 101.000
YPet in science
Pet Loss Support - Small Animal Hospital - University of Florida
All of us at the University of Florida Small Animal Hospital recognize, respect, and support the human-animal bond. We hope that after reading the material in ...
Pet Insurance University - A Vet's Guide to Pet Insurance
Are you confused about pet insurance? It is overwhelming, but you are not alone. I will walk you through the process of deciding if you need pet insurance and ...
University West Pet Clinic, 8145 University Boulevard Clive, IA ...
University West Pet Clinic is a full-service veterinary medical facility, located in Clive, IA. The professional and courteous staff at University West Pet Clinic seeks ...
Pet Tribute - College of Veterinary Medicine - Kansas State University
Mission The Pet Tribute honors the human-animal bond by supporting the College of Veterinary Medicine at Kansas State University. Making a difference ...
Welcome to Pet University Pet Information Website!
Mar 1, 2009 ... Pet University provides an assortment of articles on pet health information, pet education and pet care information.
Pet Loss Support Hotline - Tufts University
Feb 26, 2013 ... 508-839-7966. Our Hours. Messages left during off-hours will be returned, at no cost to the caller, during the next normal hotline shift.
Pet Day 2013 | College of Veterinary Medicine | Oregon State ...
... to Navigation. Oregon State University ... Pet Day is one of the most popular annual events in Corvallis! ... Flying Frisbee dogs will be smiling all over Pet Day.
Cornell University Pet Loss Support Hotline - The College of ...
Staffed by trained volunteer students from the College of Veterinary Medicine. Offers hotline hours, FAQ, and links to a variety of grief resources.
University Pet Resort - Veterinarian In Merced, CA USA :: Home
University Pet Resort - Veterinary Clinic in Merced, CA.
C.A.R.E. Pet Loss Helpline - About the Helpline, University of Illinois ...
Welcome. The Companion Animal Related Emotions Pet Loss Helpline is a confidential telephone service offered through the University of Illinois College of ...
Books on the term YPet
Reviews in Fluorescence 2008
Reviews in Fluorescence 2008
Chris D. Geddes, 2010
The CYPetYPet pair enables generation of a wide variety of high FRET efficiency protease probes. ... The improved efficiency of CYPetYPet also extends the range of substrate lengths that can be used as linkers between the fluorescent ...
Liam, Are You Sleepy Yet? [Bedtime Stories for Kids Ages 3 & Up] (Liam the Owl Series)
Liam, Are You Sleepy Yet? [Bedtime Stories for Kids Ages 3 & Up] (Liam the Owl Series)
Luis C. Lewis, 2012
#1 Amazon Best SellerBest-selling author and illustrator Luis C. Lewis shares a new delightful bedtime story about a little owl named Liam with a big imagination and a mind of his own. Liam, are you sleepy yet? Nope.Liam is having a bedtime fit! There's no way he's ready to go to bed. He's had a full day of imagined adventures and he...
Methods in bioengineering: systems analysis of biological networks
Methods in bioengineering: systems analysis of biological networks
A. Jayaraman, J. Hahn, 2009
Use EcoRI/BamHI digested and phosphatase treated pCYPet-YPet-N1 from step 13 of Section 2.3.3.3 as the vector and the gel ... The end result of this step should be the final FRET construct, pCYPet-WWElk1-YPet-N1, which contains both ...
The Best Thing Yet (McKingley)
The Best Thing Yet (McKingley)
McKenna Jeffries and Aliyah Burke, 2013
McKingley:With beautiful vistas and sights to see, come join in the journeys of our residents and visitors. They’ll embrace their desires and potentially find love that will last a lifetime. No matter where you come from…the road to enchantment leads to McKingley.The Best Thing Yet by Aliyah Burke, McKenna JeffriesBook two in the McKingley Series.I...
Handbook of Single-molecule Biophysics
Handbook of Single-molecule Biophysics
Peter. Hinterdorfer, Antoine. Van Oijen, 2009
Nevertheless, YPet is superior for single-molecule imaging because it is the brightest YFP available, with a very high ... It was based on another bright YFP variant, Topaz [63], which was the brightest YFP before the appearance of YPet.
The Attraction Factor - 4 Simple Yet Powerful Principles That Will Melt Any Man's Heart
The Attraction Factor - 4 Simple Yet Powerful Principles That Will Melt Any Man's Heart
Bob Grant, 2012
Hi, I'm Bob GrantI’m a licensed relationship expert and bestselling author who’s helped tens-of-thousands of women discover what men can't resist in a woman. In this quick and yet powerful book you're going to learn the 4 factors (or secret ingredients) that when you practice them consistently touch a man's heart in a way that m...
Fluorescent Proteins
Fluorescent Proteins
Kevin F. Sullivan, 2007
Development of an Indicator for a Protein–Protein Interaction Using CYPet and YPet Caspase-activated DNase (CAD) is ... To visualize the spatiotemporal pattern of CAD activation, we used the (GGGGS)3 linker to fuse CYPet and YPet with ...
Entwined with You: A Crossfire Novel (Crossfire Book 3)
Entwined with You: A Crossfire Novel (Crossfire Book 3)
Day, Sylvia, 2013
The sizzling third book in the #1 New York Times bestselling Crossfire Trilogy following Bared to You and Reflected in You!Praise for Sylvia Day:'Shades of Grey eclipsed by Bared to You' Evening Standard 'Tipped as the new Fifty Shades of Grey but is a lot darker and the heroine Eva is a stronger, savvy character and more likeable�...
Illuminating Spatiotemporal Regulation of Cyclic AMP Signaling ...
Illuminating Spatiotemporal Regulation of Cyclic AMP Signaling ...
Lisa M. DiPilato, 2009
This pair, named CYPet and YPet, shows a 20- fold ratiometric FRET signal change and was designed for use in ... Upon incorporation into the ICUE design, CYPet-YPet ICUE (containing the ADEP- Epacl insert) exhibited a maximum percent ...
Why You're Not Married... Yet
Why You're Not Married... Yet
McMillan, Tracy, 2012
This paperback edition includes an exclusive conversation between Bill and Giuliana Rancic, hosts of NBC’s Ready for Love, and Tracy McMillan, one of its expert matchmakers. This new relationship show features three of America’s most eligible guys searching for their soul mates. If you’re looking to get married and you’re not, there’s most likely a...
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Blog posts on the term
YPet
Autumn 2012 YPET Class graduates | The Eastern Tibet Training Institute
www.etti.org.cn/autumn-2012-ypet-class-graduates/
YPet Shop Introduces Fully Functional Wireless and in Ground Dog Fence System | Feb 6, 2013
Press Release issued Feb 6, 2013: YPet Shop now provides a complete peace of mind to all pet owners by introducing a wide variety of fully functional Wireless and In Ground Dog Fence System. Many pet owners want to let their pets roam free within certain limits and help them explore their own world in their own manner. But for many, this could be difficult to leave their pets or dogs unattended for a long time. But now YPet Shop’s Dog Fence System, people can keep their animals safe in this dangerous world.
www.sbwire.com/press-releases/ypet-shop-introduces-fully-functional-wireless-and-in-ground-dog-fence-system-203760.htm
YPet Nie - Image
my.opera.com/ypetnie/blog/2013/01/25/image-2
train ypet for sale - Abu Dhabi City - Pets For Sale, PT39782
this the most train pet in our custody now so contact for purchase at puppiesandpetss@easy.com - Pets For Sale, Abu Dhabi City, Abu Dhabi - PT39782
abu-dhabi-city.classi4uae.com/ad/pets-for-sale-train-ypet-for-sale-pid39782
ugogikup ypet ikek : Ydelyliziripyh's blog
icyzozy!ñêà÷àòüêðÿêäëÿpunishernavitel3.5crackedluxorcrackñêà÷àòücrackassassin...
ebotocyh.exblog.jp/13987711/
Quantitative FRET (Förster Resonance Energy Transfer)… | JoVE Video
A novel method involving quantitative analysis of FRET (Förster Resonance Energy Transfer) signals is described for studying enzyme kinetics.&hellip
www.jove.com/video/4430/quantitative-fret-forster-resonance-energy-transfer-analysis-for
Flintridge Family Chiropractic: The Effect of Add-On Yogic Prana Energization Technique on Healing of Fresh Fractures
Randomized control study shows exciting data:A recent article in the Journal of Alternative and Complimentary Medicine reported on a study that documented the positive effects of Yoga Prana Energization Technique (YPet) on the healing of fresh fractures. Bone fractures represent a global medical challenge for health care administrators, orthopedic care providers and patients alike, as millions of people across the world are afflicted by these injuries annually.
flintridgefamilychiropractic.blogspot.com/2011/06/effect-of-add-on-yogic-prana.html
Yoga Helps Heal Bone Fractures | Connect
This award winning yoga resource center is a complete guide for your exploration of the yoga postures, meditation techniques, pranayama, yoga therapy, and the history and philosophy of yoga.
www.yogabasics.com/connect/yoga-helps-heal-bone-fractures.html
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More cyclopaedia entries
10. YPPH
11. YPQ
12. YPR
14. YProxy
15. YPrPb
16. YPS
17. YPSL
19. YPT
20. YPT-10
23. YPT-9
24. YPTN
25. YPU
26. YPUW
27. YPW
28. YPWR
29. YPX
30. YPXM
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