Pronase
Pronase is a commercially available mixture of proteinases isolated from the extracellular fluid of Streptomyces griseus. Activity extends to both denatured and native proteins leading to complete or nearly complete digestion into individual amino acids.

This is an excerpt from the article Pronase from the Wikipedia free encyclopedia. A list of authors is available at Wikipedia.
The article Pronase at en.wikipedia.org was accessed 1,309 times in the last 30 days. (as of: 07/07/2013)
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Pronase - Wikipedia, the free encyclopedia
Pronase is a commercially available mixture of proteinases isolated from the extracellular fluid of Streptomyces griseus. Activity extends to both denatured and ...
en.wikipedia.org/wiki/Pronase
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Pronase E - Selective Proteolytic Enzymes | Sigma-Aldrich
Xaa = any amino acid residue hydrophobic = Ala, Val, Leu, Ile, Phe, Trp, Tyr ↓ = cleavage site Preferential cleavage: P6 P5 P4 P3 P2 P1 ↓ P1′ P2′ P3′ ...
www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=16410626
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SpringerProtocols: Abstract: Pronase (EC…
Pronase is the name given to a group of proteolytic enzymes that are produced in the culture supernatant of Streptomyces griseus K-1 (1–3). Pronase is known to ...
www.springerprotocols.com/Abstract/doi/10.1385/0-89603-234-5:271
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Pronase Antigen Retrieval Protocol - IHC World
Pronase Antigen Retrieval Protocol. Description: Formalin or other aldehyde fixation forms protein cross-links that mask the antigenic sites in tissue specimens , ...
www.ihcworld.com/_protocols/epitope_retrieval/pronase.htm
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53702 PRONASE ® Protease, Streptomyces griseus - EMD Millipore
Pronase ® Protease, Streptomyces griseus; EMD_BIO-53702.
www.emdmillipore.com/life-science-research/pronase-protease-streptomyces-griseus/EMD_BIO-53702/p_29Wb.s1LZV0AAAEWSmIfVhTm
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Pronase - Roche Applied Science
Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids. Resolves carboxylic acids and alcohols.
www.roche-applied-science.com/shop/custom-biotech/products/pronase
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The use of pronase in tissue culture: A comparison with trypsin
ABSTRACT. Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and.
onlinelibrary.wiley.com/doi/10.1002/jcp.1040750204/pdf
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Pronase
www.roche-applied-science.com. For life sciences research only. Not for use in diagnostic procedures. Pronase. Nuclease-free, isolated from Streptomyces ...
cssportal.roche.com/LFR_PublicDocs/ras/10165921001_en_07.pdf
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Destruction of sodium conductance inactivation in squid axons ...
We have studied the effects of the proteolytic enzyme Pronase on the membrane currents of voltage-clamped squid axons. Internal perfusion of the axons with ...
www.ncbi.nlm.nih.gov/pubmed/4755846
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Carezyme III: Pronase Kit - Biocare Medical
Pronase (Streptomyces griseus) is a commonly used digestive enzyme. In formalin- fixed paraffin-embedded tissues, certain antibody protocols require enzyme.
biocare.net/wp-content/uploads/PRT957.pdf
Search results for "Pronase"
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Pronase in science
[PDF]The use of pronase in tissue culture: A comparison with trypsin
University of Nebraska College of Medicine,. Omaha, Nebraska. ABSTRACT. Pronase is a proteolytic enzyme recently introduced as an effective dispersing ...
Pronase acutely modifies high voltage-activated calcium currents ...
Neuroscience Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada. Pronase E ('Pronase') is one of the proteolytic enzymes that are ...
Impact of pronase on flow cytometric crossmatch outcome.
Jan 21, 2011 ... Department of Medicine, Johns Hopkins University School of Medicine, Baltimore , MD 21205, USA. Pronase treatment of lymphocytes is used ...
The specificity of proteinases from Streptomyces griseus (pronase)
The specificity of proteinases from Streptomyces griseus (Pronase). Moshe Trop and Yehudith Birk. Department of Biochemistry, Bar-Ilan University, Ramat-Gan, ...
Pronase treatment facilitates alloantibody flow cytometric and ...
Department of Surgery, Indiana University, Indianapolis, IN, USA. ... Pronase, a proteolytic enzyme that targets F(c) receptors removes CD20 from B cells.
Benefit of pronase in image quality during EUS
Patients were assigned to oral premedication with saline solution (group A), Pronase and bicarbonate (group B), or Pronase, bicarbonate, and simethicone ...
Pronase-Based Assay Method for< i> O< sup> 6 -Methylguanine ...
Pronase-Based Assay Method forO6-Methylguanine–DNA Methyltransferase ... a Department of Biochemistry, School of Medicine, Ajou University, Suwon, ...
[PDF]Importance of Optimizing Pronase Treatment to Eliminate Rituximab ...
1 Clinical Transplantation Immunology Laboratory, Duke University Medical Center. 2 Tissue ... Pronase is a proteolytic enzyme that removes fragment crystal -.
Destruction of Sodium Conductance Inactivation in Squid Axons ...
Oct 1, 1973 ... The Rockefeller University Press, doi: 10.1085/jgp.62.4.375 ... At the level of a single channel, Pronase probably acts in an all-or-none manner: ...
[PDF]Destruction of Sodium Conductance Inactivation - The Journal of ...
Axons Perfused with Pronase. CLAY M. ARMSTRONG, FRANCISCO BEZANILLA , and. EDUARDO ROJAS. From the University of Rochester, School of ...
Books on the term Pronase
METHODS IN CELL BIOLOGY
METHODS IN CELL BIOLOGY
David M. Prescott, 1976
The most common procedure is to perfuse the liver briefly with Pronase (to achieve good enzyme penetration) before it is ... Such direct Pronase methods have been used by several investigators with yields of nonparenchymal liver cells ...
Molecular and Cellular Physiology of Neurons
Molecular and Cellular Physiology of Neurons
Gordon L. Fain, 1999
Pronase (x1.3) Fig. 6.9 Time course of inactivation: block by Pronase. (A) Effect of Pronase on waveform of inward Na+ current recorded from squid giant axon for 70 mV voltage pulse from VH = -70 mV to 0 mV. Axon was bathed in artificial sea  ...
RNA Methodologies: A Laboratory Guide for Isolation and ...
RNA Methodologies: A Laboratory Guide for Isolation and ...
Robert E. Farrell, Jr., 2010
Pronase (Streptomyces griseus) can be used in place of proteinase K. In some cases it may be necessary to perform a Pronase ... If necessary, this is easily accomplished by incubation of the Pronase stock (20 mg/ml in 10 mM Tris-Cl, pH 7.5; ...
Electroporation and Electrofusion in Cell Biology
Electroporation and Electrofusion in Cell Biology
E. Neumann, A.E. Sowers, C.A. Jordan, 1989
Thin horizontal bars indicate S.E.M. Also shown are the number of experiments. and stabilizing effects of Pronase E and ... In their electrofusion system, a protease inhibitor, PMSF, failed to prevent the effects of Pronase, and prior treatment of ...
Tetrahymena Thermophila
Tetrahymena Thermophila
1999
The Pronase stock is stored at —20°C. Warm the NDS + Pronase to 55°C in a water bath; this will dissolve any precipitated SDS. ... While the centrifuge is spinning, fill two labeled 1.5-ml Eppendorf tubes with 300 pl of NDS + Pronase solution.
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Blog posts on the term
Pronase
Reagents Collagenase type IV pronase E Nycodenz | Online Peptides Guide
Posted on July 2, 2013 by admin.
www.peptideslab.com/reagents-collagenase-type-iv-pronase-e-nycodenz.html
Pronase EC Sigma Aldrich digestion was achieved as described | Online Peptides Guide
Posted on May 26, 2013 by admin.
www.angiotensin-ii.com/pronase-ec-sigma-aldrich-digestion-was-achieved-as-described.html
Pronase (Molecular Biology)
The K-1 strain of Streptomyces griseus synthesizes and secretes a mixture of proteinases that for many years has been commercially available under the proprietary name of "Pronase." Initially offered as a purified proteinase of broad specificity and widely adopted for
what-when-how.com/molecular-biology/pronase-molecular-biology/
Spontaneous and pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines.
Jul 20 Spontaneous and Pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines. Related ArticlesSpontaneous and Pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines.
customantibody.ca/spontaneous-and-pronase-induced-her2-truncation-increases-the-trastuzumab-binding-capacity-of-breast-cancer-tissues-and-cell-lines/
Obstetrics & Gynecology News Article | Spontaneous and pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines |4144624
Obstetrics & Gynecology Medical Article: Spontaneous and Pronase-induced HER2 truncation increases the trastuzumab binding capacity of breast cancer tissues and cell lines
www.mdlinx.com/obstetrics-gynecology/news-article.cfm/4144624/breast-neoplasms
Internal Medicine News Article | Premedication with pronase or N-acetylcysteine improves visibility during gastroendoscopy: An endoscopist-blinded, prospective, randomized study |1751466
Internal Medicine Medical Article: Premedication with Pronase or N-acetylcysteine improves visibility during gastroendoscopy: An endoscopist-blinded, prospective, randomized study
www.mdlinx.com/internal-medicine/news-article.cfm/1751466/
Icarus Blog: Pronase, la oscura liquidación
ikarusblog.blogspot.com/2009/11/pronase-la-oscura-liquidacion.html
A Preliminary Study on the Aggregation of Porcine for Making Chimeras » Agricultural Science Paper
]]> Title A Preliminary Study on the Aggregation of Porcine for Making Chimeras Abstract Chimera is an individual with a double chromosome complement which is integrated by two or more embyos or cells with different hereditary. The formation of chimera can be achieved by two means, one is blastocyst injection, the other one is aggregation, the latter method is comparatively easier while has lower success rate.
www.agrpaper.com/a-preliminary-study-on-the-aggregation-of-porcine-for-making-chimeras.htm
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