Polymerase cycling assembly
Polymerase cycling assembly (or PCA, also known as Assembly PCR) is a method for the assembly of large DNA oligonucleotides from shorter fragments. The process uses the same technology as PCR, but takes advantage of DNA hybridization and annealing as well as DNA polymerase to amplify a complete sequence of DNA in a precise order based on the single stranded oligonucleotides used in the process. It thus allows for the production of synthetic genes and even entire synthetic genomes.

This is an excerpt from the article Polymerase cycling assembly from the Wikipedia free encyclopedia. A list of authors is available at Wikipedia.
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Polymerase cycling assembly - Wikipedia, the free encyclopedia
Polymerase cycling assembly (or PCA, also known as Assembly PCR) is a method for the assembly of large DNA oligonucleotides from shorter fragments.
en.wikipedia.org/wiki/Polymerase_cycling_assembly
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File:PCA polymerase cycling assembly.jpg - Wikipedia, the free ...
File:PCA Polymerase cycling assembly.jpg. From Wikipedia, the free encyclopedia. Jump to: navigation, search. File; File history; File usage. File:PCA ...
en.wikipedia.org/wiki/File:PCA_polymerase_cycling_assembly.jpg
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polymerase cycling assembly (PCA) - Nature
Feb 3, 2011 ... Popular Keywords: Polymerase cycling assembly (PCA) ... Circular polymerase extension cloning for high-throughput cloning of complex and ...
www.nature.com/protocolexchange/protocols?protocol_search%5Bfacets%5D%5Bkeyword%5D=polymerase+cycling+assembly+(PCA)
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Total synthesis of long DNA sequences: Synthesis of a contiguous ...
Nov 2, 2004 ... Of the various methods, Polymerase cycling assembly (PCA) (4) is the most widely used because of its inherent simplicity. Overlapping ...
www.pnas.org/content/101/44/15573.long
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Non-polymerase-cycling-assembly-based chemical gene synthesis ...
Biotechnol Adv. 2008 Mar-Apr;26(2):121-34. Epub 2007 Nov 7. Non-polymerase-cycling-assembly-based chemical gene synthesis: strategies, methods, and ...
www.ncbi.nlm.nih.gov/pubmed/18068327
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Non-polymerase-cycling-assembly-based chemical gene synthesis ...
Chemical gene synthesis is a powerful tool for basic biological research and biotechnology applications. During the last 30 years, major advances have been ...
www.sciencedirect.com/science/article/pii/S0734975007001164
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Structural Biochemistry/DNA Amplification Technique:Polymerase ...
Assembly PCR, more formally known as Polymerase cycling assembly (PCA), is a method of synthesizing long strands of DNA oligonucleotides by applying ...
en.wikibooks.org/wiki/Structural_Biochemistry/DNA_Amplification_Technique:Polymerase_Chain_Reaction_(PCR)
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polymerase cycling assembly Archives - XXPress PCR
Polymerase cycling assembly Archives | XXPress PCR - The Fastest & Most Accurate PCR Machine in the World.
www.xxpresspcr.com/tag/polymerase-cycling-assembly/
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CiteULike: Non-polymerase-cycling-assembly-based chemical gene ...
Ai-Sheng Xiong, Ri-He Peng, Jing Zhuang et al. Biotechnology Advances, Vol. 26, No. 2. (2008), pp. 121-134. blue file-import-10-01-17 heron.
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Polymerase cycling assembly Biology Terms - Tubegle
Polymerase cycling assembly Biology Terms ... Runtime: 5:39; Description: The ELM Bio Group explains the process of PCR, or Polymerase Chain Reaction.
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Polymerase cycling assembly in science
polymerase cycling assembly (PCA) - Nature
Feb 3, 2011 ... Clear. Popular Keywords: Polymerase cycling assembly (PCA) ... University of Texas Health Science Center - Houston (UTHSC-H). Post a free ...
Polymerase cycling assembly - Wikipedia, the free encyclopedia
Polymerase cycling assembly (or PCA, also known as Assembly PCR) is a method for ... Cambridge University IGEM team made a video describing the process.
Total synthesis of long DNA sequences: Synthesis of a contiguous ...
Nov 2, 2004 ... Communicated by Robert M. Stroud, University of California, San ... Of the various methods, Polymerase cycling assembly (PCA) (4) is the most ...
Circular Polymerase Extension Cloning of Complex Gene Libraries ...
J.Q was initially supported by the Duke University BME graduate program. .... polymerase cycle assembly (PCA) is used to assemble two sub-libraries into a ...
Non-polymerase-cycling-assembly-based chemical gene synthesis ...
Non-polymerase-cycling-assembly-based chemical gene synthesis: ... Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA ...
High-quality gene assembly directly from unpurified mixtures of ...
Published by Oxford University Press. .... the resulting mixture was subjected to 10 cycles of Polymerase cycling assembly (PCA, also known as recursive PCR), ...
[PDF]gene synthesis by assembly of short oligonucleotides - cIRcle
THE UNIVERSITY OF BRITISH COLUMNBIA. (Vancouver). December ...... approaches employ Polymerase cycling assembly (PCA) or Ligase Chain Reaction ...
[PDF]1 Generating a Synthetic Genome by Whole Genome Assembly ...
annealing of molecules with incorrect sequences, and 3) Assembly of ligation products into full length genomes by Polymerase cycling assembly (PCA); a non- ...
Generating a synthetic genome by whole genome assembly ...
Dec 23, 2003 ... Generating a synthetic genome by whole genome assembly: {phi} ... ligation products into full-length genomes by Polymerase cycling assembly, ... Present address: Department of Microbiology and Immunology, University of ...
Books on the term Polymerase cycling assembly
Creating Life in the Lab: How New Discoveries in Synthetic Biology ...
Creating Life in the Lab: How New Discoveries in Synthetic Biology ...
Fazal Rana, 2011
team had to carefully design the sequences of the 50 bp oligonucleotides made by chemical synthesis. 3. Performing a Polymerase cycling assembly of the paired and ligated oligonucleotides. Again, the success of this step depended on the ...
Henry's Clinical Diagnosis and Management by Laboratory Methods: Expert Consult - Online and Print, 22e
Henry's Clinical Diagnosis and Management by Laboratory Methods: Expert Consult - Online and Print, 22e
Richard A. McPherson MD and Matthew R. Pincus MD PhD, 2011
Recognized as the definitive book in laboratory medicine since 1908, Henry's Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of r...
Frontiers in Drug Design and Discovery
Frontiers in Drug Design and Discovery
Atta-ur- Rahman, Gary W. Caldwell, Barry A. Springer, 2005
Gene Synthesis by Polymerase cycling assembly (PCA) The synthetic power of the polymerase chain reaction (PCR) for the amplification nucleic acids forms one of the key technological advances from which many of the current whole gene ...
Schaum's Outline of Biochemistry, Third Edition (Schaum's Outline Series)
Schaum's Outline of Biochemistry, Third Edition (Schaum's Outline Series)
Philip Kuchel, Simon Easterbrook-Smith, Vanessa Gysbers and J. Mitchell Guss, 2011
Tough Test Questions? Missed Lectures? Not Enough Time? Fortunately for you, there's Schaum's. More than 40 million students have trusted Schaum's to help them succeed in the classroom and on exams. Schaum's is the key to faster learning and higher grades in every subject. Each Outline presents all the essential course informati...
Biotechnology fundamentals
Biotechnology fundamentals
Firdos Alam Khan, 2012
The extension time depends both on the DNA polymerase used and on the length of the DNA fragment to be amplified. As a rule-of-thumb, ... the 3'-end of the primer. 2.14.2.2 Polymerase cycling assembly Polymerase cycling assembly (PCA) ...
De novo gene synthesis by rapid polymerase chain assembly coupled with immunoaffinity purification: A novel process...
De novo gene synthesis by rapid polymerase chain assembly coupled with immunoaffinity purification: A novel process...
Joel R TerMaat, 2012
Enjoy a wide range of dissertations and theses published from graduate schools and universities from around the world. Covering a wide range of academic topics, we are happy to increase overall global access to these works and make them available outside of traditional academic databases. These works are packaged and produced by BiblioLabs under li...
Synthetic Biology: Tools and Applications
Synthetic Biology: Tools and Applications
Huimin Zhao, 2013
PCR-based assembly, otherwise known as Polymerase cycling assembly (PCA), remains one of the most cost-effective gene assembly methods.5 1'5 2 In a two- step procedure, partially overlapping oligonucleotides are designed to span the ...
The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis
The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis
Jonathan P. Clewley, 1994
The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. ...
Synthetic Biology: Computer aided design and DNA assembly
Synthetic Biology: Computer aided design and DNA assembly
Christopher Voigt, 2011
For example, oligos can be joined by Polymerase cycling assembly and subsequently amplified by PCR (Smith et al., 2003; Stemmer et al., 1995). These dsDNA fragments can then be assembled into a vector by any of the methods described ...
Fundamentals of Forensic DNA Typing
Fundamentals of Forensic DNA Typing
John M. Butler, 2009
John Butler, bestselling author of Forensic DNA Typing, now applies his expertise on the subject of DNA analysis into an introductory textbook. Fundamentals of Forensic DNA Typing walks students step-by-step through the DNA analysis process beginning with collection of evidence at a crime scene to the statistical interpretation of the results. Also...
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Blog posts on the term
Polymerase cycling assembly
NEW YORK, June 5, 2013: Polymerase Chain Reaction (PCR) In Medical Application - An Analytical Report, 2009-2015 | PRNewswire | Rock Hill Herald Online
/PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue:
www.heraldonline.com/2013/06/05/4919983/polymerase-chain-reaction-pcr.html
Market Research Reports: Medical Applications Polymerase Chain Reaction Market (PCR) 2015
The global PCR market is projected to reach around US$27. 4 billion by 2015, with a Compounded Annual Growth Rate (CAGR) of 13.
rnrmarketresearch.blogspot.com/2013/06/medical-applications-polymerase-chain.html
PR-GB.com... News from origin - Polymerase Chain Reaction (PCR) In Medical Application - An Analytical Report, 2009-2015
PR-GB.com... Read news directly from origin & Publish your PR for free...
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Polymerase Chain Reaction ~ Bio-Tech HelpLine
Polymerase Chain Reaction This is the technique developed by KRY Mullis in 1985 to amplify DNA. Through this we can generate microgram quantities of DNA copies from a single copy.
biotechhelpline16.blogspot.com/2013/02/polymerase-chain-reaction.html
Journal of Biological Engineering | Full text | 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.
www.jbioleng.org/content/7/1/2
Biotech Crunch: PCR : Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication.
biotechcrunch.blogspot.com/2012/01/pcr-polymerase-chain-reaction.html
PCR – principle, advantages and disadvantages | Medical Genetics
Medical Genetics for students
geneticamedicala.wordpress.com/2013/03/31/pcr-principle-advantages-and-disadvantages/
Polymerase Chain Reaction | j0nasthomas49
Just another WordPress.com site
j0nasthomas49.wordpress.com/2012/10/12/polymerase-chain-reaction/
Polymerase Chain Reaction- Part III: Variations or Types of PCR and Future prospects of PCR | Biotechnology | PharmaXChange.info
Polymerase Chain Reaction (PCR) is an in vitro technique based on the principle of DNA polymerization reaction by which a particular DNA sequence can be amplified and made into multiple copies.
pharmaxchange.info/press/2011/07/polymerase-chain-reaction-part-iii-variations-or-types-of-pcr-and-future-prospects-of-pcr/
polymerase chain reaction
Polymerase chain reaction From Wikipedia, the free encyclopedia http://en. wikipedia.
thepolymerasechainreaction.blogspot.com/2012/05/polymerase-chain-reaction-from.html
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